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陳紀如 Chi-Ju Chen

We sincerely invite Ph.D. program students who are interested in the studies of (1) host-virus (EBV and influenza virus) interaction and (2) DcR3-induced epithelial-mesenchymal transition (EMT) to join the lab.

Our lab currently has a technician position open for people who are interested in influenza virus research. The position is effective immediately. (posted Oct. 2009)

Fourth-year Ph.D. student, CH Ho, has been graduated this summer and becomes Dr. CH Ho after publishing his second paper in four years!  

 

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  • 專長:Molecular virology, virus/host interaction, transcription regulation
  • Office:傳醫大樓R408
  • Email:
  • Phone:886-2-2826-7180

 

Education  Research  Publications  Honors  students' Honors  Photo                         


Education

  • 1991, B.S., Department of Plant Pathology, Entomology Division, National Taiwan University, Taiwan
  • 1997, Ph.D., Genetics Program, Michigan State University, USA
  • 1997 - 2001 Post-doctoral Fellow, The Wistar Institute, University of Pennsylvania, USA
  • 2001 - 2002 Post-Doctoral Fellow (NHRI fellowship awarded) ,NHRI, Taiwan
  • 2003- 2009 Assistant Professor National Yang-Ming University, Taiwan
  • 2009 - Associate Professor National Yang-Ming University, Taiwan      

  

Research

EBV genome is packed as a chromosome-like episome at latency. Previous studies indicated that lytic replication initiates with the theta (θ)-form replication and switches to roll-circle mode after several rounds of viral DNA replication. However, the cellular and viral activities involved in the transition from histone-associated genome to θ-replication remain unclear. The first project we are interested is to identify the chromatin dynamics on EBV lytic replication origin (OriLyt). We look for the changes of histone modification (acetylation and methylation) and the factors, both cellular and viral, that are responsible for the changes.  

 

Schematic representation of the switch of EBV viral DNA replication mechanism upon reactivation    

 

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The second project we focus on is to study how decoy receptor 3 (DcR3) is regulated by exogenous factors, such as viral infection or pro-inflammatory cytokines. Both EBV and DcR3 are implicated in the development of human malignancies. DcR3 overexpression is previously demonstrated in EBV-associated lymphomas, suggesting EBV may use DcR3 as a tool to escape host immune surveillance and help EBV-infected cells gain survival advantage. We successively identified that expression of EBV lytic transactivator Rta and latent membrane protein 1 (LMP1) leads to DcR3 up-regulation. We also identified that two pro-inflammatory cytokines, TNFα and IL-6, govern DcR3 expression. The detailed mechanism of pro-inflammatory cytokines regulating DcR3 expression is under investigation. 

 

Mechanism of EBV-dependent and inflammation-associated DcR3 upregulation

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The third project we are working on is to study the association between DcR3 and chronic kidney disease (CKD). In collaboration with Dr. Der-Cherng Tarng (Department and Institute of Physiology, NYMU), we are work toward to demonstrate the function of DcR3 in CKD. We focus on the role of DcR3 in tubular epithelial-mesenchymal transition (TEMT), which has an important implication in CKD progression.


Model for the role of DcR3 in chronic kidney disease


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Publications

Chen, Chi-Ju , D. J. Leisy, and S. M. Thiem. 1996. Physical map of Anagrapha falcifera nuclear polyhedrosis virus genome. Journal of General Virology 77: 167-171.

Chen, Chi-Ju and S. M. Thiem. 1997. Differential infectivity of two Autographa californica nucleopolyhedrovirus mutants on three permissive cell lines is the result of lef-7 deletion. Virology 227: 88-95.
LINK


Chen, Chi-Ju
. 1997. Ph. D dissertation: Characterizations of baculovirus genes that influence infectivity in insect cell lines and larvae. Michigan State University

Chen, Chi-Ju
, M. E. Quentin, L. A. Brennan, C. Kukel, and S. M. Thiem. 1998. Lymantria dispar Nucleopolyhedrovirus hrf-1 expands the larval host range of Autographa californica nucleopolyhedrovirus. Journal of Virology 72: 2526-2531.
LINK

Zerby, D, C-J Chen, E. Poon, D. Lee, R. Shiekhattar, and P. M. Lieberman. 1999. The amino-terminal C/H1 domain of CREB binding protein mediates Zta transcriptional activation of latent Epstein-Barr virus. Molecular and Cellular Biology 19: 1617-1626.
LINK

Chen, Chi-Ju, Z. Deng, A. Kim, G. Blobel, and P. M. Lieberman. 2001. Stimulation of nucleosome-directed histone acetylase activity of CBP by transcriptional activators. Molecular and Cellular Biology 21:476-487.
LINK

Deng, Z.*, Chi-Ju Chen*, D. Zerby, H. Delecluse, P. M. Lieberman. 2001. Identification of acidic and aromatic residues in the Zta activation domain essential for Epstein-Barr virus reactivation. Journal of Virology 75: 10334-10347. (* co-first authors).
LINK

Deng. Z., L. Lezina, Chi-Ju Chen, S. Shtivelband, W. So, and P. M. Lieberman. 2002. Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of plasmid replication. Molecular Cell 9: 493-503. 
LINK


Deng. Z., C-J Chen, M. Chamberlin, F. Lu, G. A. Blobel, D. Speicher, L. A. Cirillo, K. S. Zaret, and P. M. Lieberman. 2003. The CBP bromodomain and nucleosome targeting are required for Zta-directed nucleosome acetylation and transcription activation. Molecular and Cellular Biology 23:2633-2644.
LINK

Hsu, CH., MD Chang, KY Tai, YT Yang, PS Wang, C-J Chen, YH Wang, SC Lee, CW Wu, and LJ Juan. 2004. HCMV IE2-mediated inhibition of HAT activity downregulates p53 function. EMBO J. 23:2269-2280.
LINK

Patel JH, Y. Du, PG Ard, C, Phillips, B Carella, C-J Chen, C. Rakowski, C. Chatterjee, PM Lieberman, WS Lan, GA Blobel, SB McMahon. 2004. The c-MYC Oncoprotein Is a Substrate of the Acetyltransferases hGCN5/PCAF and TIP60. Molecular and Cellular Biology 24:10826–10834 
LINK

 

Liu, SJ, CH Leng, SP Lien, HY Chi, CY Huang, CL Lin, WC Lian, Chi-Ju Chen, SL Hsieh, P Chong. 2006. Immunological Characterizations of the Nucleocapsid Protein Based SARS.Vaccine Candidates. Vaccine 24: 3100-3108. LINK


Ho, CH, CF Hsu, PF Fong, SK Tai, SL Hsieh, and Chi-Ju Chen*. 2007. Epstein-Barr Virus Transcription Activator Rta Upregulates Decoy Receptor 3 Expression by Binding to its Promoter. Journal of Virology 81: 4837–4847 (*corresponding author)
 LINK

 
Ho, CH, CL Chen, WY Lee, and Chi-Ju Chen*. 2009. Decoy receptor 3, upregulated by Epstein-Barr virus latent membrane protein 1, enhances nasopharyngeal carcinoma cell migration and invasion. Carcinogenesis 30:1443-1451  (*corresponding author)
LINK


Tsai SC, SJ Lin, PW Chen, WY Luo, TH Yeh, HW Wang, CJ Chen, and CH Tsai. 2009. EBV Zta protein induces the expression of interleukin-13, promoting the proliferation of EBV-infected B cells and lymphoblastoid cell lines. Blood 114: 109-118 
LINK


Kinetics of histone modification on EBV oriLyt upon reactivation. (manuscript in preparation)

Decoy Receptor 3 (DcR3) induces epithelium-mesenchymal transition in human renal cells. (manuscript in preparation)
                                                                                                              

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Honors 

  • 97學年度優良教師之教學優良獎
  • 92,93,96,97 學年度學生網路評鑑優良教師


Professional Memberships

  • American Society for Microbiology (ASM)
  • International Association for Research on EBV and Associated Diseases

Students' Honors

  • 柯佳琪 (大四) 榮獲生科系最佳畢業論文獎(2004)
  • 何政勳 (博三) 榮獲生化學會舉辦“馬武督秋令營博士/博士後研究員組”口頭競賽第一名 (2007); (博四)研究生論文比賽優等 (2009)。
  • 江長軒 (碩二) 榮獲尹珣若論文比賽優勝 (2008)

Photo


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黃國軒 (學士畢)                陳人豪 (碩士畢)    馮佩棻 (碩士畢)       張庭毓 (碩士畢)

柯佳琪 (碩士畢)                陳紀如 (PI)            陳世文 (學士畢)       江長軒 (碩士畢)

何政勳 (碩士畢,博士畢)  徐辰芳 (碩士畢)    蔡宜軒 (碩士畢)       李建緯 (碩士畢)

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