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張佩靖 Amy P. Chang

張佩靖 (Amy Pei-Ching Chang)   



Phone: 886-2-2826-7111



               國立陽明大學    藥理學研究所             碩

               國立陽明大學    生化暨分子生物研究所     博士



2016 - Present   副教授

                        國立陽明大學   微生物及免疫學研究所

2016 - Present   副研究員

                        雄醫學大學   傳染病與癌症研究中心

2015 - 2016       Visiting Scholar 

                         School of Pharmacy   University of South California (USC)

2011 - 2016       助理教授

                         國立陽明大學   微生物及免疫學研究所

2008 - 2011       博士後研究
                         Cancer Center   University of California Davis (UC Davis)

2006 - 2007       博士後研究

                         Dental Department   University of Kentucky (UK)



(碩士: 2011/09 ~ 2013/06) (論文發表: BMC Genomics. 2013 Nov 23;14:824)

張怡婷 (碩士: 2011/09 ~ 2013/06) (論文發表: PLoS One. 2014 Feb 14;9(2):e88556)

             (博士: 2014/06 ~ Present)


李松遠 (碩士: 2013/09 ~ 2015/06) (論文發表: Oncotarget. Mar 2016; accepted)
楊宛珊 (碩士: 2013/09 ~ 2015/06) (論文發表: PLoS Pathog. 2015 Jul 21;11(7):e1005051.)

       (博士: 2015/06 ~ Present)

張熒庭 (碩士: 2014/09 ~ Present)

勻里 (碩士: 2015/09 ~ Present 碩士)
黃詩晴 (共同指導,碩士: 2015/09 ~ Present 碩士)

楊佳(碩士: 2016/09 ~ Present 碩士)
李悅誠 (碩士: 2016/09 ~ Present 碩士)





Virus-Host interaction: Sumoylation, Epigenetic regulation and Cancer

   My research interest is focused on study the functional importance of sumoylation in
targeting epigenetic regulators by using KSHV as a model system. Histone methylation was thought
to be irreversible until the discovery of first histone
demethylase, LSD1, in 2004. Later on, a large
family of
JmjC-domain containing 26 members of lysine demethylases (KDMs) was identified.
Since then, Dynamic regulation of histone methylation is viewed as a driver of chromatin remodeling
and being intensively studied. By using KSHV as a model, we recently found that KDM4A is
important for maintain low levels of methylated H3K9, a favorable chromatin environment to
execute rapid transcriptional reprograming. We also found that KDM4A was targeted and

sumoylated by K-bZIP, the first viral SUMO E3 ligase we identified. The role of sumoylation in
regulating the function of KDM4A is one of the major topics in our lab. Currently, we will focus on
study the role of KDM4A in regulating IFN response and study how virus against human immune
system through targeting different KDMs. We also use KSHV as a model to study the role of the
general epigenetic regulatory role of
sumoylation in by using ChIP-Seq.


Cancer research: Autophagy signaling in Tumorigenesis

   Autophagy is a self-digestion pathway that eukaryotic cells use to degrade long-lived protein and
organelles in response to nutrition starvation or metabolic stress. Autophagy is believed to be a
double-edged sword in
oncogenesis, acting as both a tumor suppressor and a protector of cancer cell
survival. Under physiological condition, autophagy functions to protect organisms against various
pathologies, including cancer, and to promote health and longevity. On the other hand, autophagy
also emerges as a pathway to protect tumor cells from dying, and hence confers drug-resistance. In
many cancers, neuroendocrine differentiation (NED) is a process involved in
chemoresistance. Our
current focus in this topic is to study the importance and mechanism of autophagy in the

transdifferentiation process in prostate cancer (PCa). We have established a couple of autophagy-
regulated gene knockdown
PCa cell lines to study our hypothesis.


Cancer research: Non-coding RNA in Tumorigenesis

   Non-coding RNAs (ncRNAs) are RNA transcripts that are not translated into proteins. Based on
their length,
ncRNAs can be divided into two classes, short ncRNAs and long ncRNAs (lncRNAs).
ncRNA includes microRNA (miRNA) of ~20 nucleotides (nts). lncRNA are RNA transcripts
of >200
nts. LincRNAs are one class of lncRNAs that play important role in various physiology
process, such as development, metabolism, cell differentiation, and stem cells reprogramming. They
regulate gene transcription by diverse mechanisms, include (1) serve as scaffolds that recruit
transcription factor or chromatin-remodeling complex to specific genomic loci, (2) alternative
splicing, (3) translational regulation (mRNA stability), (4) act as decoys that sequestering
biomolecules, such as DNA binding proteins or
miRNAs, and therefore block their cellular function.
Alteration of
lincRNA expression in various tissue origins is identified and considered as a driving
force in
tumorigenesis. Our current focus is in identifying novel tumor related lincRNAs in PCa and
evaluation their functional role in cancer



Y.C. Lin, Y.T. Chang, M. Campbell, H.C. Lee, J. C. Shih, P.C. Chang* ”MAOA- a novel decision maker of apoptosis and autophagy in hormone refractory neuroendocrine prostate cancer cells” Scientific Reports (2017 Accept) 
Y.H. Liu, J.W. Tsai, J.L. Chen, W.S. Yang, P.C. Chang, P.L. Cheng, D.L. Turner, Y. Yanagawa, T.W. Wang, J.Y. Yu. 
“Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon.” Scientific Reports (Mar 2017)

Y.T. Chang, T.P Lin, M. Campbell, C.C. Pan, S.H. Lee, H.C. Lee, M.H. Yang, H.J. Kung, P.C. Chang* “REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer” Scientific Reports (Mar 2017)

W.S.Yang, M. Campbell, P.C. Chang* ”SUMO modification of a heterochromatin histone demethylase JMJD2A enables viral gene transactivation and viral replication.” PLoS Pathogens (Feb 2017)

P.C. Chang*, M. Campbell, Robertson ES. “Human Oncogenic Herpesvirus and Post-translational Modifications – Phosphorylation and SUMOylation” Frontiers in Microbiology (Jun 2016)

Tzu-Ping Lin,, Yi-Ting Chang, Sung-Yuan Lee, Mel Campbelld, Tien-Chiao Wang, Shu-Huei Shene, Hsiao-Jen Chungb, Yen-Hwa Changb, Allen W Chiua, Chin-Chen Panf, Chi-Hung Linb, Cheng-Ying Chuh, Hsing-Jien Kung, Chia-Yang Cheng, P.C. Chang.  
“REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling” Oncotarget (Mar  2016;  accepted)

W.S. Yang, H.W. Hsu, M. Campbell, C.Y. Cheng, P.C. Chang. “K-bZIP mediated SUMO-2/3 specific modification on the KSHV genome negatively regulates lytic gene expression and viral reactivation”  PLoS Pathogens(July 2015)

P.C. Chang and H.J. Kung. “SUMO and KSHV Replication” Cancers (Basel) (Sep 2014)

P.C. Chang, T.Y. Wang, Y.T. Chang, C.Y. Chu, C.L. Lee, H.W. Hsu, T.A. Zhou, Z. Wu, R.H. Kim, S.J. Desai, S. Liu, H.J. Kung. 
“Autophagy Pathway Is Required for IL-6 Induced Neuroendocrine Differentiation and Chemoresistance of Prostate Cancer LNCaP Cells.” PLoS One (Feb 2014) 

C.Y. Cheng, C.H. Chu, H.W. Hsu, F.R. Hsu, C.Y. Tang, W.C. Wang, H.J. Kung, P.C. Chang. 
“An improved ChIP-seq peak detection system for simultaneously identifying post-translational modified transcription factors by combinatorial fusion, using SUMOylation as an example.” BMC Genomics (Jan 2014) 

P.C. Chang, C.Y. Cheng, M. Campbell, Y.C. Yang, H.W. Hsu, T.Y. Chang, C.H. Chu, Y.W. Lee, C.L. Hung, S.M. Lai, C.G. Tepper, W.P. Hsieh, H.W. Wang, C.Y. Tang, W.C. Wang, H.J. Kung. 
“The chromatin modification by SUMO-2/3 but not SUMO-1 prevents the epigenetic activation of key immune-related genes during Kaposi's sarcoma associated herpesvirus reactivation.” BMC Genomics (Nov 2013) 

M. Campbell, K.Y. Kim, P.C. Chang, S. Huerta, B. Shevchenko, D.H. Wang, C. Izumiya, H.J. Kung, Y. Izumiya. “A Lytic Viral Long Non-coding RNA Modulates the Function of a Latent Protein” J Virol. (Nov 2013) 

M. Campbell, P.C. Chang, S. Huerta, C. Izumiya, R. Davis, C.G. Tepper, K.Y. Kim, B. Shevchenko, D.H. Wang, J.U. Jung, P.A. Luciw, H.J. Kung, Y. Izumiya. 
“Protein Arginine Methyltransferase 1-directed Methylation of Kaposi Sarcoma-associated Herpesvirus Latency-associated Nuclear Antigen” J Biol Chem. (Feb 2012) 

P.C. Chang, L.D. Fitzgerald, D.A. Hsia, Y. Izumiya, C.Y. Wu, W.P. Hsieh, S.F. Lin, M. Campbell, K.S. Lam, P.A. Luciw, C.G. Tepper, H.J. Kung 
“Histone demethylase JMJD2A regulates Kaposi's sarcoma-associated herpesvirus replication and is targeted by a viral transcriptional factor” J Virol. (Apr 2011)

P.C. Chang, Y. Izumiya, C.Y. Wu, L.D. Fitzgerald, M. Campbell, T.J. Ellison, K.S. Lam, P.A. Luciw, H.J. Kung 
“Kaposi's sarcoma associated herpesvirus (KSHV) encodes a SUMO E3 ligase which is SIM-dependent and SUMO-2/3-specific” J Biol Chem (Feb. 2010)

Z. Wu, P.C. Chang, J.C. Yang, C.Y. Chu, L.Y. Wang, N.T. Chen, A.H. Ma, S.J. Desai, S.H. Lo, C.P. Evans, K.S. Lam, and H.J. Kung “Autophagy Blockade Sensitizes Prostate Cancer Cells towards Src Family Kinase Inhibitors” Genes & Cancer (Jan. 2010)

P.C. Chang, L.D. Fitzgerald, A. Van Geelen, Y. Izumiya, T.J. Ellison, D.H. Wang, D.K. Ann, P.A. Luciw, and H.J. Kung 
“KRAB domain-associated protein-1 as a latency regulator for Kaposi’s sarcoma-associated herpesvirus and its modulation by the viral protein kinase” Cancer Res (July 2009)

P. C. Chang and M. Li “Kaposi's sarcoma-associated herpesvirus K-cyclin interacts with Cdk9 and stimulates Cdk9-mediated phosphorylation of p53 tumor suppressor” J Virol, (Jan. 2008)

Y.L. Lin, P.C. Chang, Y. Wang, and M. Li “Identification of novel viral interleukin-10 isoforms of human cytomegalovirus AD169” Virus Res, (Feb. 2008)

P. C. Chang, C.W. Chi, G.Y. Chau, F.Y. Li, Y.H. Tsai, J.C. Wu and Y.H.W. Lee “DDX3, a DEAD box RNA helicase, is deregulated in hepatitis virus associated hepatocellular carcinoma and is involved in cell growth control” Oncogene, (Mar. 2006)

W.F. Chiou, P.C. Chang, C.I. Chou, C.F. Chen “Protein constituent contributes to the hypotensive and vasorelaxant activities of Cordyceps Sinensis ” Life Sciences, (Feb. 2000)


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